Dystrobrevin alpha Antibody (OTI1B2)

Name: Dystrobrevin alpha Antibody (OTI1B2)
Brand: Novus Biologicals
SKU: NBP2-03422
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-03422

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # OTI1B2

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Product Specifications

Immunogen

Full length human recombinant protein of human DTNA (NP_116761) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

58.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dystrobrevin alpha Antibody (OTI1B2)

Western Blot: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] Analysis of extracts (35ug) from 9 different cell lines by using anti-Dystrobrevin alpha monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: Dystrobrevin alpha Antibody (OTI1B2) [NBP2-03422]

Immunocytochemistry/Immunofluorescence: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dystrobrevin alpha.

Flow Cytometry: Dystrobrevin alpha Antibody (OTI1B2) [NBP2-03422]

Flow Cytometry: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Dystrobrevin alpha antibody, and then analyzed by flow cytometry.

Western Blot: Dystrobrevin alpha Antibody (OTI1B2) [NBP2-03422]

Western Blot: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dystrobrevin alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dystrobrevin alpha.

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Applications for Dystrobrevin alpha Antibody (OTI1B2)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dystrobrevin alpha

The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].

Alternate Names

Alpha-dystrobrevin, D18S892EDTN, DRP3FLJ96209, DTN-1, DTN-2, DTN-3, DTN-A, dystrobrevin alpha, dystrobrevin, alpha, Dystrophin-related protein 3, LVNC1

Entrez Gene IDs

1837 (Human)

Gene Symbol

DTNA

Additional Dystrobrevin alpha Products

Product Documents for Dystrobrevin alpha Antibody (OTI1B2)

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Certificate of Analysis

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Product Specific Notices for Dystrobrevin alpha Antibody (OTI1B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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