E 64d

Key Product Details

Product Description

E 64d is an inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.

Product Specifications for E 64d

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for E 64d

The following data is based on the product molecular weight 342.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.60 mL	73.01 mL	146.02 mL
1 mM	2.92 mL	14.60 mL	29.20 mL
2 mM	1.46 mL	7.30 mL	14.60 mL
10 mM	0.29 mL	1.46 mL	2.92 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 38 publications citing the usage of this product.

• Hoffmann SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor. Cell 2020 PMID: 32142651
• Wilcox and Mason Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 1992 PMID: 1637341
• Sato Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 2007 PMID: 17942897
• Tanada Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 2005 PMID: 16874052
• Kim Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 2008 PMID: 18424912

Product Documents for E 64d