E. Coli O157 Antibody (9.88) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NBP2-54353AF488

Novus Biologicals

Key Product Details

Species Reactivity

E. coli

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgM Kappa Clone # 9.88
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Product Specifications

Immunogen

A crude sonicate of E. coli O157

Reactivity Notes

E. coli O157

Localization

Whole organism

Specificity

This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for E. Coli O157 Antibody (9.88) [Alexa Fluor® 488]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-54353. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: E. Coli O157

Alternate Names

H7 ompC

Additional E. Coli O157 Products

Product Documents for E. Coli O157 Antibody (9.88) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for E. Coli O157 Antibody (9.88) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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