E-Tag Epitope Tag Antibody [HRP]
Novus Biologicals | Catalog # NB600-526
Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative)
Label
HRP
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Goats were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for E-Tag Epitope Tag Antibody [HRP]
Western Blot: E-Tag Epitope Tag Antibody [HRP] [NB600-526] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).
Applications for E-Tag Epitope Tag Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:100000
Immunocytochemistry/ Immunofluorescence
1:200-1:500
Western Blot
1:1000-1:10000
Application Notes
This antibody is useful for ELISA, Western Blot and Immunocytochemistry. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody [HRP]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for E-Tag Epitope Tag Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for E-Tag Epitope Tag Antibody [HRP]
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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