E-Tag Epitope Tag Antibody - BSA Free
Novus Biologicals | Catalog # NB600-527
![Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]](https://resources.rndsystems.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0004.jpg "Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]")
Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Rabbits were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E-Tag Epitope Tag Antibody - BSA Free
Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]
Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).![Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]](https://resources.rndsystems.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0002.jpg "Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]")
Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]
Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).Applications for E-Tag Epitope Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000- 1:30000
Immunocytochemistry/ Immunofluorescence
1:100-1:400
Immunoprecipitation
1-4 ug/mg lysate
Western Blot
1:1000 - 1:30000
Application Notes
ELISA - Coating: 1:100-1:500.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for E-Tag Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for E-Tag Epitope Tag Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for E-Tag Epitope Tag Antibody - BSA Free
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Q: Re-cat # NB600-527, is this antibody the same as NB600-528, only without the HRP ?
A: NB600-527 and NB600-528 were generated by immunising rabbits with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support. In the case of NB600-528 an HRP tag was then added.
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