E2F-1 Antibody (16G7) - Azide and BSA Free

Novus Biologicals | Catalog # NB600-210

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16G7

Format

Azide and BSA Free
View all E2F-1 Primary Antibodies »

Product Specifications

Immunogen

The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This is specific for human E2F-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse E2F-1 Antibody (16G7) - Azide and BSA Free (NB600-210) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-E2F-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for E2F-1 Antibody (16G7) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210]

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210]

Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [16G7] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
E2F-1 Antibody (16G7) - Azide and BSA Free

Western Blot: E2F-1 Antibody (16G7) [NB600-210] -

Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F-1 antibody [16G7] (NB600-210) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Applications for E2F-1 Antibody (16G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E2F-1

Clone 16G7 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Long Name

E2F Transcription Factor 1

Alternate Names

E2F1, PBR3, RBAP-1, RBBP-3, RBP3

Entrez Gene IDs

1869 (Human)

Gene Symbol

E2F1

Additional E2F-1 Products

Product Documents for E2F-1 Antibody (16G7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for E2F-1 Antibody (16G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for E2F-1 Antibody (16G7) - Azide and BSA Free

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Protocols

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FAQs

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