EAAT4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13360
![Immunocytochemistry/ Immunofluorescence: EAAT4 Antibody [NBP3-13360]](https://resources.rndsystems.com/images/products/EAAT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13360-img0003.jpg "Immunocytochemistry/ Immunofluorescence: EAAT4 Antibody [NBP3-13360]")
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Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT4. The exact sequence is proprietary.
Reactivity Notes
Human (80%), Dog (81%), Bovine (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for EAAT4 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: EAAT4 Antibody [NBP3-13360]
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360]](https://resources.rndsystems.com/images/products/EAAT4-Antibody-Immunohistochemistry-Paraffin-NBP3-13360-img0002.jpg "Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360]")
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360]
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Frozen: EAAT4 Antibody [NBP3-13360]](https://resources.rndsystems.com/images/products/EAAT4-Antibody-Immunohistochemistry-Frozen-NBP3-13360-img0001.jpg "Immunohistochemistry-Frozen: EAAT4 Antibody [NBP3-13360]")
Immunohistochemistry-Frozen: EAAT4 Antibody [NBP3-13360]
Immunohistochemistry-Frozen: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.Applications for EAAT4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EAAT4
Long Name
Excitatory amino acid transporter 4
Alternate Names
SLC1A6
Gene Symbol
SLC1A6
Additional EAAT4 Products
Product Documents for EAAT4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EAAT4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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