EB3 Antibody (7J9V3)

Novus Biologicals | Catalog # NBP3-15904

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7J9V3 expressed in HEK293
View all EB3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 182-281 of human EB3 (Q9UPY8). CILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EB3 Antibody (7J9V3)

Western Blot: EB3 Antibody (7J9V3) [NBP3-15904]

Western Blot: EB3 Antibody (7J9V3) [NBP3-15904]

Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
EB3 Antibody (7J9V3)

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
EB3 Antibody (7J9V3)

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
EB3 Antibody (7J9V3)

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -

Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for EB3 Antibody (7J9V3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EB3

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Alternate Names

EB1 protein family member 3, EB3EBF3-S, End-binding protein 3, microtubule-associated protein RP/EB family member 3, microtubule-associated protein, RP/EB family, member 3, RP3EBF3APC binding protein

Gene Symbol

MAPRE3

Additional EB3 Products

Product Documents for EB3 Antibody (7J9V3)

Certificate of Analysis

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Product Specific Notices for EB3 Antibody (7J9V3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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