Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 15k8D10

Format

Azide and BSA Free
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Product Specifications

Immunogen

A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.

Specificity

The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. Clone 15K8D10 tests as being specific to the EBI3 subunit of the target protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

< 0.06 EU/ug

Scientific Data Images for EBI3 Antibody (15k8D10) - Azide and BSA Free

Western Blot: EBI3 Antibody (15k8D10)Azide Free [NBP2-27363]

Western Blot: EBI3 Antibody (15k8D10)Azide Free [NBP2-27363]

Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free [NBP2-27363] - analysis of Interleukin-35-Fc protein (A) and human Fc (B) using Interleukin-35 monoclonal antibody at 4 ug/ml.
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - Azide Free [NBP2-27363]

Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - Azide Free [NBP2-27363]

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody (15k8D10) - Azide Free [NBP2-27363] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.

Applications for EBI3 Antibody (15k8D10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

3-5ug/ml~

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

Interleukin-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, Interleukin-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which Interleukin-35 is also specifically produced. Interleukin-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

Additional EBI3 Products

Product Documents for EBI3 Antibody (15k8D10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EBI3 Antibody (15k8D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

Citations for EBI3 Antibody (15k8D10) - Azide and BSA Free

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Protocols

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FAQs

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