EBI3 Antibody (15k8D10) - BSA Free
Novus Biologicals | Catalog # NBP2-27362
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Tissue Culture Substratum
Cited:
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 15k8D10
Format
BSA Free
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Product Specifications
Immunogen
A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
Specificity
The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. Clone 15K8D10 tests as being specific to the EBI3 subunit of the target protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Endotoxin Level
< 0.06 EU/ug
Scientific Data Images for EBI3 Antibody (15k8D10) - BSA Free
![Western Blot: EBI3 Antibody (15k8D10)BSA Free [NBP2-27362]](https://resources.rndsystems.com/images/products/EBI3-Antibody-15k8D10-Tissue-Culture-Substratum-NBP2-27362-img0001.jpg "Western Blot: EBI3 Antibody (15k8D10)BSA Free [NBP2-27362]")
Western Blot: EBI3 Antibody (15k8D10)BSA Free [NBP2-27362]
Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.![Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - BSA Free [NBP2-27362]](https://resources.rndsystems.com/images/products/EBI3-Antibody-15k8D10-Immunohistochemistry-Paraffin-NBP2-27362-img0002.jpg "Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - BSA Free [NBP2-27362]")
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - BSA Free [NBP2-27362]
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.Applications for EBI3 Antibody (15k8D10) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 31601798)
Immunohistochemistry
reported in scientific literature (PMID 27681506)
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
3-5ug/ml~
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EBI3
Long Name
Epstein-Barr virus induced gene 3
Alternate Names
EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3
Gene Symbol
EBI3
UniProt
Additional EBI3 Products
Product Documents for EBI3 Antibody (15k8D10) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EBI3 Antibody (15k8D10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for EBI3 Antibody (15k8D10) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Th1 Differentiation Pathway
