Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Rabbit IgG
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 180-228 of mouse EBI3 was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for EBI3 Antibody [Allophycocyanin]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EBI3

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

Additional EBI3 Products

Product Documents for EBI3 Antibody [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EBI3 Antibody [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EBI3 Antibody [Allophycocyanin]

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  • Q: I have purchased the following Immunohistochemsitry anti mouse antibodies from you: NBP2-03944APC and NBP2-25196F. Can u advise how much to use of each per slide? 

    A: You can see the recommended dilutions listed on the unconjugated antibody page and then re-calculate them to adjust for the difference in the concentration between the unconjugated and conjugated antibody. For NBP2-03944APC, the unconjugated base can be found here. For NBP2-25196F, the unconjugated antibody base is here. The concentrations of your conjugates can be found on the vial.

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