EDEM2 Antibody (2E4) - Azide and BSA Free

Novus Biologicals | Catalog # H00055741-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E4

Format

Azide and BSA Free
View all EDEM2 Primary Antibodies »

Product Specifications

Immunogen

C20orf31 (NP_060687, 479 a.a. ~ 578 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PIDPAALHCCQRLKEEQWEVEDLMREFYSLKRCRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSKLALLGQVFLDSS

Reactivity Notes

Human. Other species not tested.

Specificity

C20orf31 - chromosome 20 open reading frame 31

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EDEM2 Antibody (2E4) - Azide and BSA Free

Western Blot: EDEM2 Antibody (2E4) [H00055741-M01]

Western Blot: EDEM2 Antibody (2E4) [H00055741-M01]

Western Blot: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody (M01), clone 2E4.Lane 1: EDEM2 transfected lysate(64.8 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01]

Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01]

Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
ELISA: EDEM2 Antibody (2E4) [H00055741-M01]

ELISA: EDEM2 Antibody (2E4) [H00055741-M01]

ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged C20orf31 is approximately 30ng/ml as a capture antibody.
Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01]

Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01]

Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDEM2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/ml as a capture antibody.

Applications for EDEM2 Antibody (2E4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EDEM2

In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM]

Alternate Names

bA4204.1, C20orf31, C20orf49, chromosome 20 open reading frame 31, ER degradation enhancer, mannosidase alpha-like 2, ER degradation-enhancing alpha-mannosidase-like 2, ER degradation-enhancing-mannosidase-like protein 2, FLJ10783

Entrez Gene IDs

55741 (Human)

Gene Symbol

EDEM2

UniProt

NP_060687 (Human)

Additional EDEM2 Products

Product Documents for EDEM2 Antibody (2E4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EDEM2 Antibody (2E4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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