EDF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84011

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EDF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDF1 Antibody - BSA Free

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human lymph node showing strong nuclear and cytoplasmic postitivity in geminal and non-geminal center cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human duodenum shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human skin showing nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human cerebellum showing strong nuclear and cytoplamic positivity in Purkinje cells.
EDF1 Antibody - BSA Free Western Blot: EDF1 Antibody - BSA Free [NBP1-84011]

Western Blot: EDF1 Antibody - BSA Free [NBP1-84011]

Analysis in human cell line MCF-7.
EDF1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: EDF1 Antibody - BSA Free [NBP1-84011]

Immunocytochemistry/ Immunofluorescence: EDF1 Antibody - BSA Free [NBP1-84011]

Staining of human cell line U-2 OS shows localization to cytosol.

Applications for EDF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EDF1

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Gene Symbol

EDF1

Additional EDF1 Products

Product Documents for EDF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EDF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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