EDF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92882

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EDF1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1). MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQRRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDF1 Antibody - BSA Free

Western Blot: EDF1 AntibodyBSA Free [NBP2-92882]

Western Blot: EDF1 AntibodyBSA Free [NBP2-92882]

Western Blot: EDF1 Antibody [NBP2-92882] - Analysis of extracts of various cell lines, using EDF1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Rat brain using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Human gastric cancer using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody - BSA Free [NBP2-92882]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Mouse lung using EDF1 antibody at dilution of 1:100 (40x lens).
Knockout Validated: EDF1 Antibody - BSA Free [NBP2-92882]

Western Blot: EDF1 Antibody - BSA Free [NBP2-92882]

Western Blot: EDF1 Antibody [NBP2-92882] - Analysis of extracts from normal (control) and EDF1 Rabbit pAb knockout (KO) NIH/3T3 cells, using EDF1 Rabbit pAb antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for EDF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EDF1

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Gene Symbol

EDF1

Additional EDF1 Products

Product Documents for EDF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EDF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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