EEA1 Antibody (6D4.1D4) - BSA Free
Novus Biologicals | Catalog # NBP2-36568
![Western Blot: EEA1 Antibody (6D4.1D4)BSA Free [NBP2-36568]](https://resources.rndsystems.com/images/products/EEA1-Antibody-6D4-1D4-Western-Blot-NBP2-36568-img0006.jpg "Western Blot: EEA1 Antibody (6D4.1D4)BSA Free [NBP2-36568]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Virus
Predicted:
Bat (93%), Porcine (95%), Primate (99%), Rat (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6D4.1D4
Format
BSA Free
Loading...
Product Specifications
Immunogen
Partial recombinant human EEA1 protein (between amino acids 50-300). [UniProt Q15075]
Localization
Cytoplasm. Early endosome membrane.
Marker
Early Endosome Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for EEA1 Antibody (6D4.1D4) - BSA Free
Western Blot: EEA1 Antibody (6D4.1D4)BSA Free [NBP2-36568]
Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]](https://resources.rndsystems.com/images/products/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0004.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]")
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.![Flow (Intracellular): EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]](https://resources.rndsystems.com/images/products/EEA1-Antibody-6D4-1D4-Flow-Intracellular-NBP2-36568-img0002.jpg "Flow (Intracellular): EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]")
Flow (Intracellular): EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]
Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.![Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]](https://resources.rndsystems.com/images/products/EEA1-Antibody-6D4-1D4-Immunohistochemistry-Paraffin-NBP2-36568-img0005.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]")
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - BSA Free [NBP2-36568]
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.Applications for EEA1 Antibody (6D4.1D4) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
2.5 ug/million cells
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 33212210)
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5-10 ug/ml
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EEA1
Long Name
Early Endosome Antigen 1
Alternate Names
MST105, MSTP105, ZFYVE2
Gene Symbol
EEA1
UniProt
Additional EEA1 Products
Product Documents for EEA1 Antibody (6D4.1D4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EEA1 Antibody (6D4.1D4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for EEA1 Antibody (6D4.1D4) - BSA Free
Powered by Bioz
Customer Reviews for EEA1 Antibody (6D4.1D4) - BSA Free
There are currently no reviews for this product. Be the first to review EEA1 Antibody (6D4.1D4) - BSA Free and earn rewards!
Have you used EEA1 Antibody (6D4.1D4) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...