EEA1 Antibody (M347)

Name: EEA1 Antibody (M347)
Brand: Novus Biologicals
SKU: NBP3-23301
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23301

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M347

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Product Specifications

Immunogen

Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1. [UniProt# Q15075]

Specificity

This antibody detects a 180 kDa protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EEA1 Antibody (M347)

Western Blot: EEA1 Antibody (M347) [NBP3-23301] -

Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).

Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] -

Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.

Applications for EEA1 Antibody (M347)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EEA1

EEA1 binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Gene Symbol

EEA1

Additional EEA1 Products

Product Documents for EEA1 Antibody (M347)

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Certificate of Analysis

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Product Specific Notices for EEA1 Antibody (M347)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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