EEA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30914

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunomicroscopy, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EEA1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.

Localization

Cytoplasm, Early endosome membrane

Marker

Early Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEA1 Antibody - BSA Free

Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug each lane of HEK-293 cell extracts. Image from verified customer review.
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma. Antibody at 1:500.
Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914] - Mouse brain. 5% SDS PAGE. Antibody at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract. 5% SDS-PAGE. Antibody at 1:1000.
Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914]

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug whole cell lysate/extracts B. Antibody at 1:5000.
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody [NBP1-30914]

Immunocytochemistry/ Immunofluorescence: EEA1 Antibody [NBP1-30914]

Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa cells, using EEA1 (NBP1-30914) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled red.
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914]

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
EEA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EEA1 Antibody [NBP1-30914] -

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (NBP1-30914) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for EEA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunomicroscopy

Reported in scientific literature (PMID: 27795427)

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-30914 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEA1

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Entrez Gene IDs

8411 (Human)

Gene Symbol

EEA1

UniProt

Q15075 (Human)

Additional EEA1 Products

Product Documents for EEA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EEA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for EEA1 Antibody - BSA Free

Customer Reviews for EEA1 Antibody - BSA Free (1)

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  • EEA1 Antibody
    Name: Luiz Alexandre Magno
    Application: Western Blot
    Sample Tested: HEK 293 whole cell lysate
    Species: Human
    Verified Customer | Posted 05/05/2019
    3 samples from HEK-293 (30 ug each) incubated with the EEA-1 antibody NBP1-30914. Bands appear on top of the 150 kDa molecular weight and are very specific for these cells.
    Antibody concentration: 1:1,000 Incubation time/temp: overnight at 4C (shaking)
    EEA1 Antibody - BSA Free NBP1-30914

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