EEF1D Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91861

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1D Antibody - BSA Free

Immunohistochemistry-Paraffin: EEF1D Antibody [NBP1-91861]

Immunohistochemistry-Paraffin: EEF1D Antibody [NBP1-91861]

Immunohistochemistry-Paraffin: EEF1D Antibody [NBP1-91861] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
Western Blot: EEF1D Antibody [NBP1-91861]

Western Blot: EEF1D Antibody [NBP1-91861]

Western Blot: EEF1D Antibody [NBP1-91861] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
EEF1D Antibody - BSA Free Western Blot: EEF1D Antibody - BSA Free [NBP1-91861]

Western Blot: EEF1D Antibody - BSA Free [NBP1-91861]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
EEF1D Antibody - BSA Free Western Blot: EEF1D Antibody - BSA Free [NBP1-91861]

Western Blot: EEF1D Antibody - BSA Free [NBP1-91861]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for EEF1D Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1D

EEF1D encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Antigen NY-CO-4, EF-1D, EF-1-delta, EF1DFP1047, elongation factor 1-delta, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein), FLJ20897, guanine nucleotide exchange protein

Gene Symbol

EEF1D

Additional EEF1D Products

Product Documents for EEF1D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EEF1D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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