EEF1E1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92227

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all EEF1E1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1). MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1E1 Antibody - Azide and BSA Free

Western Blot: EEF1E1 AntibodyAzide and BSA Free [NBP2-92227]

Western Blot: EEF1E1 AntibodyAzide and BSA Free [NBP2-92227]

Western Blot: EEF1E1 Antibody [NBP2-92227] - Analysis of extracts of various cell lines, using EEF1E1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: EEF1E1 Antibody - Azide and BSA Free [NBP2-92227]

Immunocytochemistry/ Immunofluorescence: EEF1E1 Antibody - Azide and BSA Free [NBP2-92227]

Immunocytochemistry/Immunofluorescence: EEF1E1 Antibody [NBP2-92227] - Analysis of L929 cells using EEF1E1 at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for EEF1E1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:200-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF1E1

EEF1E1 encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, ARS-interacting multifunctional protein 3, Elongation factor p18, eukaryotic translation elongation factor 1 epsilon 1, eukaryotic translation elongation factor 1 epsilon-1, Multisynthase complex auxiliary component p18, P18, p18 component of aminoacyl-tRNA synthetase complex

Gene Symbol

EEF1E1

Additional EEF1E1 Products

Product Documents for EEF1E1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EEF1E1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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