EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25446

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2541 expressed in HEK293

Format

Azide and BSA Free
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Synthtic peptide of human EGFR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free (NBP3-25446) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] -

Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI.
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Western Blot: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Immunohistochemistry-Paraffin: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.
Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI.*Competitor's antibody is not affiliated with Novus and does not endorse this product.
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Western Blot: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

*The competitor is not affiliated with Novus and does not endorse this product.
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Western Blot: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

*The competitor is not affiliated with Novus and does not endorse this product.
EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant [NBP3-25446] -

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI.*Competitor's antibody is not affiliated with Novus and does not endorse this product.

Applications for EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGFR

Epidermal growth factor receptor (EGFR), also known as ErbB1 and HER1, is a type I glycoprotein that belongs the ErbB subfamily of receptor tyrosine kinases (RTKs), which includes ErbB2/HER2, ErbB3/HER3, and ErbB4/HER4 (1,2). EGFR plays an important role in epithelial cell development and homeostasis and as a driver of tumorigenesis in cancer (1,2). The human EGFR is protein 1210 amino acids (aa) in length with a theoretical molecular weight (MW) of 134 kDa (1). The protein consists of a short signal peptide, an extracellular domain (ECD) divided into four subdomains (I-IV), a transmembrane region, an intracellular juxtamembrane segment, a tyrosine kinase domain, and C-terminal tail (1-3). Within the ECD, human EGFR has 88-90% aa sequence identity with mouse and rat EGFR. EGFR has four known specific ligands: EGF, amphiregulin, epigen, and transforming growth factor alpha (TGF-alpha). EGFR ligands betacellulin, epiregulin, and herapin binding (HB)-EGF have dual specificity with ErbB4 (1,3). Ligand binding to the extracellular domain of EFGR leads to receptor homodimerization, or heterodimerization with other ErbB family members, and EGFR activation. This results in subsequent phosphorylation and activation of intracellular signaling pathways, such as MAPK and PI3K/Akt (2,3). EGFR signaling is essential for many cellular processes including proliferation, differentiation, migration, and apoptosis (1,3,5).

In addition to its role in normal development, EGFR mutations or overexpression is observed in many tumors, including breast cancer, non-small cell lung carcinoma (NSCLC), colon cancer, and more (3-6). Small molecule tyrosine kinase inhibitors (TKIs), like gefitinib, erlotinib, and afatinib, have shown great efficacy in treating patients with EGFR activating mutations, especially for NSCLC (4-6). However, most patients eventually develop acquired resistance to TKIs and thus combination and alternative therapies are in development (4-6). A third-generation TKI, osimertinib, is approved for NSCLC patients with resistance to first-line EGFR TKI treatment (6). Additionally, combination therapies of EGFR TKIs with monoclonal antibody immunotherapies, like anti-PD-L1, are being further investigated in clinical trials (6).

References

1. Roskoski R Jr. Small molecule inhibitors targeting the EGFR/ErbB family of protein-tyrosine kinases in human cancers. Pharmacol Res. 2019; 139:395-411. https://doi.org/10.1016/j.phrs.2018.11.014

2. Sigismund S, Avanzato D, Lanzetti L. Emerging functions of the EGFR in cancer. Mol Oncol. 2018; 12(1):3-20. https://doi.org/10.1002/1878-0261.12155

3. Normanno N, De Luca A, Bianco C, et al. Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 2006; 366(1):2-16. https://doi.org/10.1016/j.gene.2005.10.018

4. Liu Q, Yu S, Zhao W, Qin S, Chu Q, Wu K. EGFR-TKIs resistance via EGFR-independent signaling pathways. Mol Cancer. 2018; 17(1):53. https://doi.org/10.1186/s12943-018-0793-1

5. Harrison PT, Vyse S, Huang PH. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. Semin Cancer Biol. 2020; 61:167-179. https://doi.org/10.1016/j.semcancer.2019.09.015

6. Wu SG, Shih JY. Management of acquired resistance to EGFR TKI-targeted therapy in advanced non-small cell lung cancer. Mol Cancer. 2018; 17(1):38. https://doi.org/10.1186/s12943-018-0777-1

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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