EGR2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-16286
![Western Blot: EGR2 Antibody [NBP2-16286]](https://resources.rndsystems.com/images/products/EGR2-Antibody-Western-Blot-NBP2-16286-img0001.jpg "Western Blot: EGR2 Antibody [NBP2-16286]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Rat
Predicted:
Porcine (95%), Rat (90%), Rhesus Macaque (98%), Xenopus (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human EGR2. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (95%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EGR2 Antibody - BSA Free
Western Blot: EGR2 Antibody [NBP2-16286]
Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.![Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286]](https://resources.rndsystems.com/images/products/EGR2-Antibody-Immunohistochemistry-Frozen-NBP2-16286-img0002.jpg "Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286]")
Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286]
Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using antibody at 1:500 dilution.
Western Blot: EGR2 Antibody [NBP2-16286] -
Western Blot: EGR2 Antibody [NBP2-16286] - Sample (30 ug of whole cell lysate)A: zebrafish brain
10% SDS PAGE
NBP2-16286 diluted at 1:1000
Immunohistochemistry: EGR2 Antibody [NBP2-16286] -
Immunohistochemistry: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EGR2 antibody (NBP2-16286) at 1:300 dilution.Applications for EGR2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID:35151688).
Immunohistochemistry
Assay dependent
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EGR2
Alternate Names
AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20
Gene Symbol
EGR2
UniProt
Additional EGR2 Products
Product Documents for EGR2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EGR2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for EGR2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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