EHD1 Antibody (5S4P9)

Name: EHD1 Antibody (5S4P9)
Brand: Novus Biologicals
SKU: NBP3-16201
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16201

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5S4P9 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9). MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EHD1 Antibody (5S4P9)

Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201]

Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201] - Western blot analysis of extracts of various cell lines, using EHD1 Rabbit mAb (NBP3-16201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201]

Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201] - Immunohistochemistry of paraffin-embedded human appendix using EHD1 Rabbit mAb (NBP3-16201) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for EHD1 Antibody (5S4P9)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EHD1

The EHD1 gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be a

Alternate Names

EH-domain containing 1

Gene Symbol

EHD1

Additional EHD1 Products

Product Documents for EHD1 Antibody (5S4P9)

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Certificate of Analysis

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Product Specific Notices for EHD1 Antibody (5S4P9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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