EIF3B Antibody (S09-2A9)

Novus Biologicals | Catalog # NBP3-15026

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-2A9 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide of human EIF3B (Uniprot#: P55884)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF3B Antibody (S09-2A9)

Western Blot: EIF3B Antibody (S09-2A9) [NBP3-15026]

Western Blot: EIF3B Antibody (S09-2A9) [NBP3-15026]

Western Blot: EIF3B Antibody (S09-2A9) [NBP3-15026] - Western blot detection of EIF3B in K562, Hela cell lysates using EIF3B Rabbit mAb (1:1000 diluted). Predicted band size: 93kDa. Observed band size: 117kDa.
Immunohistochemistry-Paraffin: EIF3B Antibody (S09-2A9) [NBP3-15026]

Immunohistochemistry-Paraffin: EIF3B Antibody (S09-2A9) [NBP3-15026]

Immunohistochemistry-Paraffin: EIF3B Antibody (S09-2A9) [NBP3-15026] - Immunohistochemistry of EIF3B in paraffin-embedded Human breast cancer tissue using EIF3B Rabbit mAb at dilution 1/20

Applications for EIF3B Antibody (S09-2A9)

Application
Recommended Usage

Immunohistochemistry

1/20-1/50

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF3B

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Alternate Names

eIF3 p110, eIF3 p116, eIF3b, eIF-3-eta, EIF3-P110, EIF3-P116, EIF3S9EIF3-ETA, Eukaryotic translation initiation factor 3 subunit 9, eukaryotic translation initiation factor 3 subunit B, eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD), eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa, eukaryotic translation initiation factor 3, subunit B, MGC104664, MGC131875, Prt1 homolog, PRT1hPrt1

Gene Symbol

EIF3B

Additional EIF3B Products

Product Documents for EIF3B Antibody (S09-2A9)

Certificate of Analysis

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Product Specific Notices for EIF3B Antibody (S09-2A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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