EIF3J Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04592

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all EIF3J Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2). MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF3J Antibody - Azide and BSA Free

Western Blot: EIF3J AntibodyAzide and BSA Free [NBP3-04592]

Western Blot: EIF3J AntibodyAzide and BSA Free [NBP3-04592]

Western Blot: EIF3J Antibody [NBP3-04592] - Analysis of extracts of various cell lines, using EIF3J antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
EIF3J Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] -

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] - Immunofluorescence analysis of PC-12 cells using EIF3J Rabbit pAb (A8637) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3J Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] -

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] - Immunofluorescence analysis of HeLa cells using EIF3J Rabbit pAb (A8637) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3J Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] -

Immunocytochemistry/ Immunofluorescence: EIF3J Antibody - Azide and BSA Free [NBP3-04592] - Immunofluorescence analysis of NIH/3T3 cells using EIF3J Rabbit pAb (A8637) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for EIF3J Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EIF3J

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM]

Alternate Names

eIF3 p35, eIF3-alpha, eIF3j, eIF3-p35, EIF3S1eIF-3-alpha, Eukaryotic translation initiation factor 3 subunit 1, eukaryotic translation initiation factor 3 subunit J, eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD), eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa, eukaryotic translation initiation factor 3, subunit J

Gene Symbol

EIF3J

Additional EIF3J Products

Product Documents for EIF3J Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EIF3J Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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