eIF4ENIF1 Antibody

Name: eIF4ENIF1 Antibody
Brand: Novus Biologicals
SKU: NB100-1368
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-1368

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-AKVISVDELEYRQ corresponding to C-Terminus according to NP_062817.2, NP_001157974.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for eIF4ENIF1 Antibody

Western Blot: eIF4ENIF1 Antibody [NB100-1368]

Western Blot: eIF4ENIF1 Antibody [NB100-1368] - Analysis of eIF4ENIF1 (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry: eIF4ENIF1 Antibody [NB100-1368]

Immunohistochemistry: eIF4ENIF1 Antibody [NB100-1368] - IHC analysis of eIF4ENIF1 in paraffin embedded Human Pancreas using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for eIF4ENIF1 Antibody

Application

Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.25 - 0.5 ug/ml

Application Notes

WB: Approx. 140-150 kDa band observed in 293 lysates (predicted MW of approx. 108 kDa band according to NP_062817.1) and is in agreement with scientific literature (Dostie et al). IHC-P: Human pancreas shows strong staining of cytoplasm in acinar cells-.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF4ENIF1

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic;its own nuclear import is regulated by a nuclear localization signal and nuclear export signals.

Alternate Names

4E-TFLJ26551,2610509L04Rik, Clast4, eIF4E-transporter, eukaryotic translation initiation factor 4E nuclear import factor 1eIF4E transporter, eukaryotic translation initiation factor 4E transporter, FLJ21601

Entrez Gene IDs

56478 (Human); 74203 (Mouse)

Gene Symbol

EIF4ENIF1

UniProt

NP_062817. (Human)

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Product Specific Notices for eIF4ENIF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for eIF4ENIF1 Antibody

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