Elastin Antibody (BA-4)

Novus Biologicals | Catalog # NB100-2076

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine, Feline, Rabbit

Cited:

Human, Rat, Porcine, Feline, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BA-4
View all Elastin Primary Antibodies »

Product Specifications

Immunogen

Bovine alpha-Elastin.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 30214669). Feline reactivity reported in scientific literature (PMID:33091431

Localization

Connective tissue.

Specificity

This recognizes insoluble elastin, alpha-elastin, soluble non-cross linked precursor of elastin (tropoelastin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Elastin Antibody (BA-4) (NB100-2076) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Elastin Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Elastin Antibody (BA-4)

Western Blot: Elastin Antibody (BA-4) [NB100-2076]

Western Blot: Elastin Antibody (BA-4) [NB100-2076]

Western Blot: Elastin Antibody (BA-4) [NB100-2076] - Analysis of tropoelastin using the mAb BA-4 elastin antibody (NB100-2076). A: Western blot of tropoelastin purified from bovine ligamentum nuchae tissue minces. B: Autoradiogram of a gradient SDS-polyacrylamide gel showing [3H] leucine-labeled tropoelasti.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human renal cell carcinoma stained with Elastin antibody (NB100-2076)
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Elastin surrounding blood vessel in pig testis. Image from verified customer review.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Analysis of elastin using clone BA-4 (NB100-2076) in formalin-fixed, paraffin embedded human first trimester decidua. H&E counterstain. Original magnification X100.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076] - Formalin fixed paraffin embedded human kidney stained with Elastin antibody.
Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Immunohistochemistry-Paraffin: Elastin Antibody (BA-4) [NB100-2076]

Elastin-Antibody-BA-4-Immunohistochemistry-Paraffin-NB100-2076-img0007.jpg
Elastin Antibody (BA-4)

Immunocytochemistry/ Immunofluorescence: Elastin Antibody (BA-4) [NB100-2076] -

Immunocytochemistry/ Immunofluorescence: Elastin Antibody (BA-4) [NB100-2076] - Presence of ECM proteins collagen, elastin, fibronectin, vitronectin & GAGs following decellurarization.Porcine vena cavas were decellularized & analyzed for ECM proteins. (A) Cross-section of paraffin embedded samples were immunostained for collagen type I/III, elastin, fibronectin & vitronectin. Images were taken at identical microscopy settings. Scale bar is 100 μm. (B-E) Quantification of ECM components with commercially available kits from 20–30 mg wet tissue (B) soluble collagen, (C) insoluble collagen, (D) elastin, & (E) GAGs. Fluorescence intensity of samples immunostained for (F) fibronectin & (G) vitronectin. The fluoresvence intensity was quantified with ImageJ, & the values were normalized to the untreated group. n = 5 for all groups. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31381614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Elastin Antibody (BA-4)

Application
Recommended Usage

Immunohistochemistry

1:25-1:50

Western Blot

1:100-1:2000
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30214669).. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:33091431).

Reviewed Applications

Read 2 reviews rated 3.5 using NB100-2076 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Elastin

Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Alternate Names

elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS

Entrez Gene IDs

2006 (Human)

Gene Symbol

ELN

UniProt

P15502 (Human)

Additional Elastin Products

Product Documents for Elastin Antibody (BA-4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Elastin Antibody (BA-4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Elastin Antibody (BA-4)

Customer Reviews for Elastin Antibody (BA-4) (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Elastin Antibody (BA-4)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 11/04/2017
    Blue-DAPI, Red-NB100-2076 used 1:100 to detect Elastin. The image was captured using an epifluorescent microscopy.
    Elastin Antibody (BA-4) NB100-2076
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Pig testis FFPE
    Species: Other
    Verified Customer | Posted 07/07/2014
    Elastin surrounding blood vessel in pig testis
    Elastin Antibody (BA-4) NB100-2076

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