ELF3/ESE-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87945

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ELF3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMA

Reactivity Notes

Mouse (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELF3/ESE-1 Antibody - BSA Free

Western Blot: ELF3/ESE-1 Antibody [NBP1-87945]

Western Blot: ELF3/ESE-1 Antibody [NBP1-87945]

Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-ELF3 antibody. Corresponding ELF3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
ELF3/ESE-1 Antibody - BSA Free Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody - BSA Free [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody - BSA Free [NBP1-87945]

Analysis in human colon and skeletal muscle tissues using HPA003479 antibody. Corresponding ELF3 RNA-seq data are presented for the same tissues.
ELF3/ESE-1 Antibody - BSA Free Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody - BSA Free [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody - BSA Free [NBP1-87945]

Staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon, endometrium, pancreas and skeletal muscle using Anti-ELF3/ESE-1 antibody NBP1-87945 (A) shows similar protein distribution across tissues to independent antibody NBP1-87946 (B).
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human pancreas shows moderate nuclear positivity in pancreatic ducts.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945]

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Applications for ELF3/ESE-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ELF3

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

Long Name

E74-like factor 3

Alternate Names

EPR1, ERT, ESX, JEN

Entrez Gene IDs

1999 (Human)

Gene Symbol

ELF3

UniProt

P78545

Additional ELF3 Products

Product Documents for ELF3/ESE-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ELF3/ESE-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ELF3/ESE-1 Antibody - BSA Free

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  • Q: Do you have any data to indicate that it works for ChIP, and if not are you willing to provide 8 ug for a trial ChIP - we will give shipping charges.

    A:

    We have not tested this antibody for its use in ChIP. While we can not provide you a trial sample, if you would like to test this antibody in an untested application and share your results with us, then I can recommend our Innovators Reward Program.

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