Emerin Antibody (EMD/2167)

Novus Biologicals | Catalog # NBP2-79784

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # EMD/2167
View all Emerin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary) (Uniprot: P50402)

Localization

Nuclear Membrane

Marker

Papillary Thyroid Carcinoma and EDMD Marker

Specificity

Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79918)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Emerin Antibody (EMD/2167)

Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784]

Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784]

Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Antibody (EMD/2167).
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Antibody (EMD/2167).
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Antibody (EMD/2167).
Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784]

Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784]

Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Emerin Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Emerin Antibody (EMD/2167)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-3 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

UniProt

P50402 (Human)

Additional Emerin Products

Product Documents for Emerin Antibody (EMD/2167)

Certificate of Analysis

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Product Specific Notices for Emerin Antibody (EMD/2167)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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