EMMPRIN/CD147 Antibody (BSG/9223R)
Novus Biologicals | Catalog # NBP3-23674
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # BSG/9223R
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Product Specifications
Immunogen
Recombinant full-length human EMMPRIN/CD147 protein
Localization
Cell surface.
Specificity
This monoclonal antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for EMMPRIN/CD147 Antibody (BSG/9223R)
![EMMPRIN/CD147 Antibody (BSG/9223R) Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -](https://resources.rndsystems.com/images/products/nbp3-23674_rabbit-emmprin-cd147-mab-bsg-9223r-2412025874816.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -")
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] - Formalin-fixed, paraffin-embedded human placenta stained with EMMPRIN/CD147 Antibody (BSG/9223R). Inset: PBS instead of primary antibody; secondary only negative control.![EMMPRIN/CD147 Antibody (BSG/9223R) Western Blot: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -](https://resources.rndsystems.com/images/products/nbp3-23674_rabbit-emmprin-cd147-mab-bsg-9223r-26202521345245.jpg "Western Blot: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -")
Western Blot: EMMPRIN/CD147 Antibody (BSG/9223R) [NBP3-23674] -
Western Blot Analysis of HepG2 cell lysate using EMMPRIN/CD147 Antibody (BSG/9223R).Applications for EMMPRIN/CD147 Antibody (BSG/9223R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EMMPRIN/CD147
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
Additional EMMPRIN/CD147 Products
Product Documents for EMMPRIN/CD147 Antibody (BSG/9223R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EMMPRIN/CD147 Antibody (BSG/9223R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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