EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70278

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI9H5

Format

Azide and BSA Free
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD147 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD147.
Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD147.
Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Staining of paraffin-embedded Human Kidney tissue using anti-CD147 mouse monoclonal antibody.
Flow Cytometry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Flow Cytometry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Flow Cytometry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CD147 antibody, and then analyzed by flow cytometry.
Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Analysis of extracts (10ug) from 10 Human tissue by using anti-BSG monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5)Azide and BSA Free [NBP2-70278]

Western Blot: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CD147 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Staining of HT29 cells using anti-CD147 mouse monoclonal antibody.
Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278]

Immunohistochemistry: EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free [NBP2-70278] - Staining of paraffin-embedded Human prostate tissue using anti-CD147 mouse monoclonal antibody.

Applications for EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1500-3000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EMMPRIN/CD147 Antibody (OTI9H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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