Enolase 1 Antibody (1G7) - BSA Free

Novus Biologicals | Catalog # NBP2-59457

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G7

Format

BSA Free
View all Enolase 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Enolase 1 (1-434aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Enolase 1 Antibody (1G7) - BSA Free

Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]

Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]

Flow Cytometry: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]

Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Lane 1: PC3 cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: HeLa cell lysates
Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]

Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ENO1 (Alpha-enolase) recombinant proteinLane 2.: ENO2 (Gamma-enolase) recombinant proteinLane 3.: ENO3 (Beta-enolase) recombinant protein

Applications for Enolase 1 Antibody (1G7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1059

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 1

Enolase 1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody (1G7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Enolase 1 Antibody (1G7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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