ENTPD4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81510

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ENTPD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FGGNAARQRYEDRIFANTIQKNRLLGKQTGLTPDMPYLDPCLPLDIKDEIQQNGQTIYLRGTGDFDLCRETIQPFMNKTNETQTSLNGVYQPPIHFQNSEFYGFSEFYYCTEDVLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENTPD4 Antibody - BSA Free

Western Blot: ENTPD4 Antibody [NBP1-81510]

Western Blot: ENTPD4 Antibody [NBP1-81510]

Western Blot: ENTPD4 Antibody [NBP1-81510] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunocytochemistry/ Immunofluorescence: ENTPD4 Antibody [NBP1-81510]

Immunocytochemistry/ Immunofluorescence: ENTPD4 Antibody [NBP1-81510]

Immunocytochemistry/Immunofluorescence: ENTPD4 Antibody [NBP1-81510] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: ENTPD4 Antibody [NBP1-81510]

Immunohistochemistry-Paraffin: ENTPD4 Antibody [NBP1-81510]

Immunohistochemistry-Paraffin: ENTPD4 Antibody [NBP1-81510] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for ENTPD4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENTPD4

Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP, AMP, ADP, ATP and UMP are not substrates. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP

Alternate Names

ectonucleoside triphosphate diphosphohydrolase 4, KIAA0392NTPDase 4, LALP70EC 3.6.1.6, LAP70guanosine-diphosphatase like protein, LYSAL1uridine-diphosphatase, lysosomal apyrase-like 1, Lysosomal apyrase-like protein of 70 kDa, NTPDase-4, UDPaseapyrase, lysosomal, Uridine-diphosphatase

Gene Symbol

ENTPD4

Additional ENTPD4 Products

Product Documents for ENTPD4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ENTPD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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