Designed to isolate human eosinophils by high affinity negative selection. The column eluate represents a highly enriched eosinophil population with minimal contamination of either CD16+ neutrophils or lymphocytes.
Prepared suspensions of polymorphonuclear granulocytes are incubated with a mixture of monoclonal antibodies and then loaded onto the Eosinophil Enrichment Column. Neutrophils of non-interest and contaminating lymphocytes bind to anti-immunoglobulin (Ig) coated glass beads by F(ab)-surface Ig interactions or by non-specific Fc interactions. The column eluate contains a highly enriched eosinophil population containing < 1% lymphocytes. Recovery ranges between 10 - 30% of all available eosinophils loaded. The purity of recovered eosinophils ranges from 90 to 97%.
Generates samples of 90 – 97% purified eosinophils
Separation solution for enhanced isolation of polymorphonuclear cells (PMN)
Gradient solution for the depletion of red blood cells (RBCs)
2 Eosinophil Enrichment Columns
Monoclonal Antibody Cocktail
10X Column Buffer
RBC Gradient Solution
PMN Separation Solution
Eosinophil Enrichment Column Principle. Purification of eosinophil populations by high affinity negative selection.
Figure 2. (A) Immunophenotyping analysis of human PMNs prior to being loaded onto Eosinophil Enrichment Columns. The frequency of eosinophils, CD16 dull, and CD49d positive cells in the starting population, is approximately 3.4% of loaded cells. (B) CD16-FITC and CD49d-PE staining analysis of cells eluted from the Eosinophil Enrichment Columns. The eosinophil purity of the recovered cell population is approximately 98%.