EphA2 Antibody (66G9) - BSA Free

Novus Biologicals | Catalog # NBP2-59479

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 66G9

Format

BSA Free
View all EphA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human EPHA2 (27-537aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for EphA2 Antibody (66G9) - BSA Free

Western Blot: EphA2 Antibody (66G9)BSA Free [NBP2-59479]

Western Blot: EphA2 Antibody (66G9)BSA Free [NBP2-59479]

Western Blot: EphA2 Antibody (66G9) [NBP2-59479] - Lane 1: Recombinant human EPHA2 extracellular domain, Lane 2: Recombinant human EPHA2 cytoplasmic domain
Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (66G9) - BSA Free [NBP2-59479]

Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (66G9) - BSA Free [NBP2-59479]

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (66G9) [NBP2-59479] - Analysis of EPHA2 in HeLa cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of EPHA2 in A431 cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for EphA2 Antibody (66G9) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 1 using NBP2-59479 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphA2

Eph receptor A2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Alternate Names

Eck, Myk2, Sek2

Gene Symbol

EPHA2

Additional EphA2 Products

Product Documents for EphA2 Antibody (66G9) - BSA Free

Certificate of Analysis

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Product Specific Notices for EphA2 Antibody (66G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EphA2 Antibody (66G9) - BSA Free (2)

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  • EphA2 Antibody (66G9) - BSA Free
    Name: Grace Stroman
    Application: Immunoprecipitation
    Sample Tested: PDAC whole cell lysates
    Species: Human
    Verified Customer | Posted 02/13/2023
    EPHA2 IP comparing CST and Novus NBP2-59479 antibodies. Input = whole cell lysate; Non-precipitated = protein not bound to antibody-magnetic bead complex; Eluate = protein bound to antibody-magnetic bead complex
    EphA2 Antibody (66G9) - BSA Free NBP2-59479
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • EphA2 Antibody (66G9)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse lens tissue
    Species: Mouse
    Verified Customer | Posted 07/28/2021
    Anterior Posterior section of mouse lens stained with Mouse Anti-EphA2 antibody, Phalloidin-Rhodamine, and DAPI. The imaging revealed that there was no signal in neither wild-type nor knock-out samples.
    EphA2 Antibody (66G9) - BSA Free NBP2-59479
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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