Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # IIIA4
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Product Specifications

Immunogen

The original antibody was prepared by intraperitoneal immunization of a BALB/c mouse with LK63 cells (Uniprot#: P29320)

Specificity

The antibody binds to the native EphA3 globular ephrin-binding domain. The antibody targets a site closely adjacent to the heterotetramerization site on the N-terminal of EPHA3's extracellular domain adjacent to the ligand-binding site and has a high affinity for EPHA3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This is a chimeric rabbit antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for EphA3 Antibody (IIIA4) - Chimeric

EphA3 Antibody (IIIA4) - Chimeric EphA3 Antibody (IIIA4) - Chimeric

Immunocytochemistry/ Immunofluorescence: EphA3 Antibody (IIIA4) - Chimeric [NBP3-48887] -

Immunocytochemistry/ Immunofluorescence: EphA3 Antibody (IIIA4) - Chimeric [NBP3-48887] - Immunofluorescence analysis of paraformaldehyde-fixed LNCaP cells on coverslips stained with NBP3-48887 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing weak membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-48887, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for EphA3 Antibody (IIIA4) - Chimeric

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.

Surface Plasmon Resonance

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphA3

Eph receptor A3 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Long Name

Eph Receptor A3

Alternate Names

Cek4, Hek4, Tyro4

Additional EphA3 Products

Product Documents for EphA3 Antibody (IIIA4) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EphA3 Antibody (IIIA4) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EphA3 Antibody (IIIA4) - Chimeric

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  • Q: Can we use NBP2-16346  for IP?

    A:

    We have not validated EphA3 Antibody NBP2-16346 for IP so we do not know for certain if it will work. If you decide to buy and test it you can take advantage of our Innovators Rewards Program for unvalidated applications.

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