EphB1 Antibody

Novus Biologicals | Catalog # NBP3-12305

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all EphB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 850-900 on mouse Eph BR1 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for EphB1 Antibody

Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305]

Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305]

Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305] - Mouse Brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305]

Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305]

Immunohistochemistry-Paraffin: EphB1 Antibody [NBP3-12305] - Mouse Brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X magnification.

Applications for EphB1 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:750

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB1

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Gene Symbol

EPHB1

Additional EphB1 Products

Product Documents for EphB1 Antibody

Certificate of Analysis

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Product Specific Notices for EphB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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