Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E5

Format

BSA Free
View all Epoxide Hydrolase 1/EPHX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human EPHX1 (21-455aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for EPHX1 Antibody (2E5) - BSA Free

Western Blot: EPHX1 Antibody (2E5)BSA Free [NBP1-30163]

Western Blot: EPHX1 Antibody (2E5)BSA Free [NBP1-30163]

Western Blot: EPHX1 Antibody (2E5) [NBP1-30163] - The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody (2E5) - BSA Free [NBP1-30163]

Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody (2E5) - BSA Free [NBP1-30163]

Immunocytochemistry/Immunofluorescence: EPHX1 Antibody (2E5) [NBP1-30163] - Analysis of EPHX1 in Hep3B cells line, stained withDAPI (Blue) for nucleus staining and monoclonal anti-humanEPHX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor488 conjugate (Green).
Western Blot: EPHX1 Antibody (2E5)BSA Free [NBP1-30163]

Western Blot: EPHX1 Antibody (2E5)BSA Free [NBP1-30163]

Western Blot: EPHX1 Antibody (2E5) [NBP1-30163] - The cell lysates (10ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanEPHX1 (1:500). Proteins were visualized using a goat antimousesecondary antibody conjugated to HRP and an ECLdetection system.Lane 1 : 293T cell lysateLane 2 : EPHX1 transfected 293T cell lysate

Applications for EPHX1 Antibody (2E5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Epoxide Hydrolase 1/EPHX1

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

EPHX, EPHX1, EPOX, Epoxide Hydratase, HYL1, MEH, MEH8, Microsomal Epoxide Hydrolase

Entrez Gene IDs

2052 (Human)

Gene Symbol

EPHX1

UniProt

NP_000111

Additional Epoxide Hydrolase 1/EPHX1 Products

Product Documents for EPHX1 Antibody (2E5) - BSA Free

Certificate of Analysis

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Product Specific Notices for EPHX1 Antibody (2E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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