To induce and evaluate EMT, the biological process implicated in CSC generation and metastasis.
Epithelial to mesenchymal transition (EMT) is critical for normal embryonic development, and aberrantly reactivated EMT mechanisms in adults contribute to pathological states such as metastasis. Growing evidence indicates that EMT mechanisms contribute to cancer stem cell (CSC) generation, suggesting that therapeutic targeting of EMT may restrict CSCs and inhibit migratory behavior associated with metastasis.
To reliably induce EMT in vitro in only 5 days using a defined formulation.
To assess EMT status in human cells using three fluorochrome-conjugated antibodies.
Fluorescence-based assays for investigating chemotaxis, cell migration, and cell invasion.