epithelial Sodium Channel gamma Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18256
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Hamster, Xenopus
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
Localization
Apical Cell Membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for epithelial Sodium Channel gamma Antibody - BSA Free
Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256]
Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Western blot analysis of Rat kidney tissue lysates showing detection of epithelial Sodium Channel gamma protein using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:1000.Immunohistochemistry: epithelial Sodium Channel gamma Antibody [NBP3-18256]
Immunohistochemistry: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Immunohistochemistry analysis using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256]
Western Blot: epithelial Sodium Channel gamma Antibody [NBP3-18256] - Western blot analysis of Mouse kidney cortex showing detection of epithelial Sodium Channel gamma protein using Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256). Primary Antibody: Rabbit Anti-epithelial Sodium Channel gamma Polyclonal Antibody (NBP3-18256) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.Applications for epithelial Sodium Channel gamma Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: epithelial Sodium Channel gamma
Long Name
Amiloride-sensitive sodium channel subunit gamma
Alternate Names
ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G
Gene Symbol
SCNN1G
Additional epithelial Sodium Channel gamma Products
Product Documents for epithelial Sodium Channel gamma Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for epithelial Sodium Channel gamma Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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