EPS15R Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84828

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EPS15R Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQMISSLKTQIQSQESDLKSQEDDLNRAKSELNRLQQEETQLEQSIQAGRVQLETIIKSLKSTQDEINQARSKLSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EPS15R Antibody - BSA Free

Immunohistochemistry-Paraffin: EPS15R Antibody [NBP1-84828]

Immunohistochemistry-Paraffin: EPS15R Antibody [NBP1-84828]

Immunohistochemistry-Paraffin: EPS15R Antibody [NBP1-84828] - Staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.
EPS15R Antibody - BSA Free Western Blot: EPS15R Antibody - BSA Free [NBP1-84828]

Western Blot: EPS15R Antibody - BSA Free [NBP1-84828]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EPS15L1 antibody. Remaining relative intensity is presented.

Applications for EPS15R Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPS15R

EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Evidence suggests that eps15 and eps15R are involved in endocytosis (1). The most striking difference between these two related proteins is that Eps15R is also found in the nucleus, whereas Eps15 is excluded from this compartment at steady state (2). Eps15R plays a key role in clathrin-mediated endocytosis of transmembrane receptors. Using a mutational approach, it has been found that the second ubiquitin-interacting motif (UIM) of Eps15 and Eps15R is essential for their ubiquitination (3)

Alternate Names

epidermal growth factor receptor pathway substrate 15-like 1, epidermal growth factor receptor substrate 15-like 1, epidermal growth factor receptor substrate EPS15R, Eps15R, Eps15-related protein

Gene Symbol

EPS15L1

Additional EPS15R Products

Product Documents for EPS15R Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EPS15R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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