Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08501

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Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CS1-4

Format

Azide and BSA Free
View all Epstein Barr Virus Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing the sequence of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP

Reactivity Notes

EBV

Localization

Nuclear

Specificity

This antibody is a mixture of four different monoclonal antibodies. This antibody is specific to 60kDa latent membrane protein (LMP-1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic C-terminus of the cytoplasmic domain of LMP-1. This antibody stains strongly with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. EBV, also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07676).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

Immunohistochemistry-Paraffin: Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free [NBP3-08501]

Immunohistochemistry-Paraffin: Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free [NBP3-08501]

Immunohistochemistry-Paraffin: Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free [NBP3-08501] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Epstein Barr Virus Mouse Monoclonal Antibody (CS1-4).

Applications for Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Epstein Barr Virus

Epstein Barr virus (EBV) is a member of the herpesvirus family and one of the most common human viruses. Most people become infected with EBV during their lives. Primary infections usually results in infectious mononucleosis (glandular fever) but the virus can also lay dormant in B lymphocytes and when reactivated become associated with more serious disease such as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease.

Alternate Names

Epstein Barr Virus, Human herpesvirus type 4, Hypothetical protein BALF1

Additional Epstein Barr Virus Products

Product Documents for Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Epstein Barr Virus Antibody (CS1-4) - Azide and BSA Free

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  • Q: I need the names of the primary and secondary antibodies used in an EBV immunohistochemical method.

    A: We have the following primary antibodies for EBV that have been validated in IHC. You would then choose a secondary dependent on the host of the primary you choose - anti-rat or anti-mouse - and conjugated to the label of your choice.

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