ER alpha/NR3A1 Antibody (ER506)

Novus Biologicals | Catalog # NBP2-33320

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ER506
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Product Specifications

Immunogen

Recombinant fragment (around aa 2-185) of human ER alpha/NR3A1 protein (exact sequence is proprietary) (Uniprot: P03372)

Localization

Nucleus

Marker

Marker of Estrogen Dependence

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33321)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER alpha/NR3A1 Antibody (ER506)

Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).
Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).
Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.
Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]

Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for ER alpha/NR3A1 Antibody (ER506)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 and T47D lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 65 kDa.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ER alpha/NR3A1

The estrogen receptor (ER) is a ~ 67kDa protein, originally described by Jensen and Jacobson, 1962 as a uterine protein which bound 3H-estradiol with high affinity. More than two decades later the cDNA encoding the human estrogen receptor was finally cloned and sequenced. The estrogen receptor gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. The A/B domain of the estrogen receptor contains a constitutively active transcription activation function called TAF-1. Domain C contains two Cys4 zinc fingers, which form the core of the DNA binding domain; it also contains a weak constitutive dimerization activity. The zinc fingers interact with DNA, and the ER homodimer appears to bind most tightly to the palindromic estrogen response element (ERE) sequence GGATCNNNGATCC. The hinge domain D appears to be the location for binding by heat shock proteins. The estrogen receptor is an important regulator of growth and differentiation in the mammary gland. Estrogens play an important role also in breast cancer development, although tumors often progress towards an autocrine growth. This hormone-independence and the incomplete correlation between ER-status and response to endocrine (tamoxifen) therapy may be explained by the presence of receptor variants with either constitutive transcriptional activity or a dominant negative inhibition of normal ER function. Many investigators have reported on the isolation of cDNAs which encode variant ER sequences. Many of these variants appear to be formed by errors in RNA splicing which result in one or more exons being lost during processing of the ER message.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

UniProt

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (ER506)

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (ER506)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail