ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]

Novus Biologicals | Catalog # NBP2-33321AF594

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Protein Array, CyTOF-ready

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ER506
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 2-185) of human ER alpha/NR3A1 protein (exact sequence is proprietary) (Uniprot: P03372)

Localization

Nucleus

Marker

Marker of Estrogen Dependence

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]

ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]

Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594] [NBP2-33321AF594] -

Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594] [NBP2-33321AF594] - Six color intervals for tumor sorting analysis were utilized with ER-alpha 594 set in the 605-laser channel. Positive controls and positive group were both successfully identified. Image from a verified customer review.

Applications for ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-33321AF594 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594] (1)

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Customer Images

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  • ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human lung cancer cell line
    Species: Human and Mouse
    Verified Customer | Posted 02/07/2024
    We utilized six color intervals for tumor sorting analysis, with ER-alpha 594 set in the 605 laser channel. The detection results were satisfactory, with positive controls and our positive group both successfully identified.
    ER alpha/NR3A1 Antibody (ER506) [Alexa Fluor® 594] NBP2-33321AF594

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail