ER alpha/NR3A1 Antibody (EVG F9)

Novus Biologicals | Catalog # NB100-74413

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Key Product Details

Species Reactivity

Human, Mouse, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EVG F9
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) & E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9582378).

Specificity

Estrogen Receptor alpha (EVG F9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ER alpha/NR3A1 Antibody (EVG F9)

Western Blot: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Analysis of various whole cell lysates.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in U251 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Cancer biopsies of deparaffinized Human breast carcinoma tissues.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in Hela Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in MCF-7 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] -

Normal deparaffinized Human uterus tissue.

Applications for ER alpha/NR3A1 Antibody (EVG F9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:2000
Application Notes
WB: Detects an approx. 68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (EVG F9)

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (EVG F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail