ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-45004

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM567

Format

IHC-Prediluted
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ER alpha/NR3A1 protein (aa2-185) (Uniprot: P03372)

Localization

Nuclear

Marker

Marker of Estrogen Dependence

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted

ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted Western Blot: ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted [NBP2-45004] -

Western Blot: ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted [NBP2-45004] -

Western Blot: ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted [NBP2-45004] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).

Applications for ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (SPM567) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail