ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34801

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM567

Format

Azide and BSA Free
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ER alpha/NR3A1 protein (aa2-185) (Uniprot: P03372)

Localization

Nuclear

Marker

Marker of Estrogen Dependence

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32965).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

Western Blot: ER alpha/NR3A1 Antibody (SPM567)Azide and BSA Free [NBP2-34801]

Western Blot: ER alpha/NR3A1 Antibody (SPM567)Azide and BSA Free [NBP2-34801]

Western Blot: ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).
ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] -

Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] - Analysis using the Alexa Fluor (R) 594 conjugate of NBP2-34801.Clusters of CAF markers were used to identify the fibroblast population in mice tumor model. The ER-alpha expressing patterns in the small group were checked. The Fluor is 594, which was set as BV 605 in BD machine. Image from verified customer review.

Applications for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.

Immunohistochemistry-Paraffin

1-2ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail