ER beta/NR3A2 Antibody
Novus Biologicals | Catalog # NB120-3577
![Western Blot: ER beta/NR3A2 Antibody [NB120-3577]](https://resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-Western-Blot-NB120-3577-img0005.jpg "Western Blot: ER beta/NR3A2 Antibody [NB120-3577]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Hamster, Primate, Sheep
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Gel Super Shift Assays
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 16153501). Bovine reactivity reported in scientific literature (PMID: 21397601). Hamster reactivity reported in scientific literature (PMID: 12943744).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ER beta/NR3A2 Antibody
Western Blot: ER beta/NR3A2 Antibody [NB120-3577]
Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.![Immunocytochemistry/ Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577]](https://resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3577-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577]")
Immunocytochemistry/ Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577]
Immunocytochemistry/Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577] - Primate amygdala.![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577]](https://resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-Immunohistochemistry-Paraffin-NB120-3577-img0004.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577] - Figure 1 illustrates immunolocalization of ER beta in primate amygdala using NB120-3577.Applications for ER beta/NR3A2 Antibody
Application
Recommended Usage
Gel Super Shift Assays
1 - 5 ul
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1 - 2 ug/ml
Immunohistochemistry-Frozen
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
1 - 2 ug/ml
Application Notes
WB: Detects an approx. 55 - 62 kDa protein representing ER beta from rat brain homogenate. ChIP usage was reported in the scientific literature (PMID: 19897598).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ER beta/NR3A2
Long Name
Estrogen Receptor beta
Alternate Names
ESR2, NR3A2
Gene Symbol
ESR2
UniProt
Additional ER beta/NR3A2 Products
Product Documents for ER beta/NR3A2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ER beta/NR3A2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ER beta/NR3A2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
