ERAB Antibody (OTI10B4)

Novus Biologicals | Catalog # NBP2-02118

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10B4
View all ERAB Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSD17B10(NP_004484) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ERAB Antibody (OTI10B4) (NBP2-02118) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ERAB Antibody (OTI10B4)

Western Blot: ERAB Antibody (10B4) [NBP2-02118] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunocytochemistry/ Immunofluorescence: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunocytochemistry/Immunofluorescence: ERAB Antibody (10B4) [NBP2-02118] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAB.
Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded pancreas tissue using anti-ERABmouse monoclonal antibody.
Flow Cytometry: ERAB Antibody (OTI10B4) [NBP2-02118]

Flow Cytometry: ERAB Antibody (OTI10B4) [NBP2-02118]

Flow Cytometry: ERAB Antibody (10B4) [NBP2-02118] - Analysis of Hela cells, using anti-ERAB antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: ERAB Antibody (OTI10B4) [NBP2-02118]

Western Blot: ERAB Antibody (OTI10B4) [NBP2-02118]

Western Blot: ERAB Antibody (10B4) [NBP2-02118] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAB.
Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERAB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-ERAB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Kidney tissue using anti-ERAB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (OTI10B4) [NBP2-02118]

Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded liver tissue using anti-ERABmouse monoclonal antibody.

Applications for ERAB Antibody (OTI10B4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERAB

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Alternate Names

17-beta-HSD 10, 17-beta-hydroxysteroid dehydrogenase 10, 17b-HSD10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, ABAD, amyloid-beta peptide binding alcohol dehydrogenase, CAMR, DUPXp11.22, EC 1.1.1.178, EC 1.1.1.35, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, ERAB3-hydroxyacyl-CoA dehydrogenase type-2, HADH2AB-binding alcohol dehydrogenase, hydroxyacyl-Coenzyme A dehydrogenase, type II, hydroxyacyl-Coenzyme Adehydrogenase, type II, hydroxysteroid (17-beta) dehydrogenase 10, mental retardation, X-linked, syndromic 10, MHBD, Mitochondrial ribonuclease P protein 2,3-hydroxyacyl-CoA dehydrogenase type II, Mitochondrial RNase P protein 2, MRPP2HCD2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, short chain dehydrogenase/reductase family 5C, member 1, short chain L-3-hydroxyacyl-CoA dehydrogenase type 2, short chain type dehydrogenase/reductase XH98G2, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, XH98G2

Entrez Gene IDs

837 (Human)

Gene Symbol

CASP4

Additional ERAB Products

Product Documents for ERAB Antibody (OTI10B4)

Certificate of Analysis

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Product Specific Notices for ERAB Antibody (OTI10B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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