ErbB2/Her2 Antibody (HRB2/282)

Novus Biologicals | Catalog # NBP2-32863

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HRB2/282
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant extracellular domain of human ErbB2/Her2 protein (Uniprot: P04626)

Localization

Cell Surface

Specificity

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This monoclonal antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34642)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ErbB2/Her2 Antibody (HRB2/282)

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/282) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863] - Flow Cytometric Analysis of trypsinized PFA-fixed MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/282); followed by Goat anti-mouse IgG-CF488 (Blue); Isotype control (Red).
Protein Array: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Protein Array: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863]

Protein Array: ErbB2/Her2 Antibody (HRB2/282) [NBP2-32863] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/282). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for ErbB2/Her2 Antibody (HRB2/282)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

UniProt

P04626

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (HRB2/282)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody (HRB2/282)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ErbB2/Her2 Antibody (HRB2/282)

Customer Reviews for ErbB2/Her2 Antibody (HRB2/282)

There are currently no reviews for this product. Be the first to review ErbB2/Her2 Antibody (HRB2/282) and earn rewards!

Have you used ErbB2/Her2 Antibody (HRB2/282)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (HRB2/282)

Showing  1 - 2 of 2 FAQs Showing All

    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

    • A: The concentration is 0.2 mg/ml.
Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies