ErbB2/Her2 Antibody (rERBB2/6637)

Novus Biologicals | Catalog # NBP3-23581

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2b Kappa Clone # rERBB2/6637
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encoding the extracellular domain of human ErbB2/Her2

Localization

Cell Surface

Specificity

This monoclonal antibody is specific and shows minimal cross-reaction with other members of the EGFR-family.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for ErbB2/Her2 Antibody (rERBB2/6637)

ErbB2/Her2 Antibody (rERBB2/6637) Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] -

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] -

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Stained using ErbB2/Her2 Antibody (rERBB2/6637) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
ErbB2/Her2 Antibody (rERBB2/6637) Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] -

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] -

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ErbB2/Her2 Antibody (rERBB2/6637) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for ErbB2/Her2 Antibody (rERBB2/6637)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (rERBB2/6637)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ErbB2/Her2 Antibody (rERBB2/6637)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (rERBB2/6637)

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    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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